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KMID : 0364819880260030188
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Korean Journal of Microbiology
1988 Volume.26 No. 3 p.188 ~ p.196
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Protoplast Fusion of Lactose Assimilating Yeasts
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Abstract
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1
Intergeneric or lntraspeeif c protoplast fusion between Saccharomyces cerevisiae X2100-IA, Candida pseudotropkals ATCC $619 and CBS 607 was attempted to produce ethanol from lactose containing materials. The intergenerk fusion frequency between Saccharomyces cerevisiae X21$0?IA (ade rho) and Candida pseudotropkalis CBS 607 (his met) was 1.0 x 10-5. These values exhibited approximately 2-3.5 fold increase when compared with fusion frequency obtained without the treatment of bovine serum albumin, myoinositol and ergosterol, suggesting that these compounds may Improve intergenerie or intaspecific protophst fusion. Nuclear fusion appears to occur in fusants between intergenera(S. cerevirlae + C pseudotropkaiis) and latraspecles (C. pseudotropkaiis strains) as strongly suggested by DNA content, nuclear staining, comparison of survival rate to UV light and isolation of recombinants after mitotic segregation. It was also found that alcohol production from intraspecific hybrids was somewhat Increased when compared with that from their parents.
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KEYWORD
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